2013年11月18日 美国消息，亚利桑那州--PCR快速检测EMS/AHPND成为可能。
亚利桑那大学对虾病理实验室Dr. Donald Lightner团队的助理研究员Linda Nunan给对虾养殖从业者发布了以下信息：
亲爱的对虾养殖业同仁：
我代表亚利桑那大学技术转让办公室发布以下声明：当前可应用PCR技术检测AHPND。
急性肝胰脏坏死病（AHPND）引起中国、越南、泰国和马来西亚养殖对虾大量死亡。自2009年该病被首次报道以来，该疾病造成损失超过10亿美元/年。亚利桑那大学研究人员已经发现AHPND由定植于对虾肠道的细菌所引起，其可对宿主肝胰腺等消化器官造成组织破坏。研究人员发现AHPNS病原体为常见海洋细菌——副溶血性弧菌的一种特殊变异菌株。该菌株具有一个1.8万对的碱基序列，且仅在病原分离物中发现，故其可作为PCR检测AHPND的目标基因序列。
当前，应用组织学鉴定是判断对虾是否感染AHPND的唯一方法，该方法耗时长，费用高。应用PCR技术开展快速病原检测技术将迅速投入市场，促进对虾产业发展。该目标序列具有独特性，在NCBI数据库中不存在同源匹配序列。伴随进口对虾比例占美国总消费量比如攀升至85％以上，快速检测AHPND技术对海鲜市场将有着极高价值。
技术优势：
实现AHPND的快速检测；
检测成本低，且无需组织学分析；
大批量检测成为可能。
November 18, 2013
United States
Arizona—PCR Detection of Acute Hepatopancreatic Necrosis Disease (AHPND/EMS) Available!!!
Linda Nunan, Assistant Staff Scientist at Dr. Donald Lightner’s shrimp pathology laboratory at the University of Arizona, posted the following information to The Shrimp List, a mailing list for the shrimp farming industry:
“Dear shrimp list people:
I am posting the following for the Office of Technology Transfer at the University of Arizona. The technology for PCR detection of AHPND is now available.
Acute Hepatopancreatic Necrosis Disease (AHPND) is causing high mortalities in farm-raised shrimp cultured in China, Vietnam, Thailand and Malaysia. Since the disease was first reported in 2009, it now causes annual losses of more than $1 billion. Faculty at the University of Arizona have found that AHPND is caused by a bacterial agent which colonizes the intestinal tract of shrimp and causes tissue destruction of the shrimp digestive organ known as the hepatopancreas. The inventors found the AHPNS pathogen is a unique strain of the common marine bacterium Vibrio parahaemolyticus. The unique strain possess an 18 kilo-base pair gene sequence that is only present in the pathogenic isolate, so it can be a target for detection of AHPND using a PCR assay.
Currently, the only method to determine if a shrimp is infected with AHPND causing bacterium is through the use of histology, which is time consuming and expensive. A rapid PCR test for the detection of this pathogen will be the first on the market and will be beneficial to the shrimp producing industry. The targeted sequence is also unique; there are no homologous matches to any other sequences in the NCBI database. With imports of shrimp into USA upwards of 85% of total consumption, quick detection of AHPND could be very valuable to the seafood market.
Advantages
- Rapid detection of AHPND
-Cost effective without the need for histology
-Able to test much larger quantities of shrimp”
